Methods to crystallize RNA.
نویسندگان
چکیده
Preparation of suitably large and well-ordered single crystals is usually the rate-limiting step in the determination of the three-dimensional structure of RNAs and their complexes with proteins by X-ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNA-protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.
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عنوان ژورنال:
- Current protocols in nucleic acid chemistry
دوره Chapter 7 شماره
صفحات -
تاریخ انتشار 2001